LOCUS       sequence-96523-e        3835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_11
FEATURES             Location/Qualifiers
     source          1..3835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1960..2059)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2076..2095)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2077..2095)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2100..2116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2100..2116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2218..2237)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2229..3038
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2296..2315)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3185..3773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3674..3693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcacctg agagcggatt ttacagatgt catgtgagtt
      721 cattgagtaa tattcttatg taccactact gactgcttgg gactttatta ctgtaaatag
      781 gaaaaacaaa aaattaagat tctgatatgc taattatttt gcttatatgg tggtgaatat
      841 acttttaaaa tgaaacatgt attgcatgca acaatactat tttggtagat ttttagaaaa
      901 gcaggtatta ttacaggtaa taggcaataa tgacttcgaa ggtcttaggg actatgagta
      961 ggtaaacact ttatgaccac gaccccacaa acaccccgca ggagattatg tggctgccat
     1021 ttgtaaacgg ccattcattg tcctgtctct tgcctttggc tctttccagt ttttcttccc
     1081 aaatctttgg actagttcct gcagaaaata tgaattcaca gtggtgccct ctgggcctgg
     1141 gcatctctgg gccggcagac cctgagcagc gactgctgct gagagcaggt gtgtgtacag
     1201 atggcatggt ggggccagcc tgccttctgc ttggtgatgt tggagagggt gattgataat
     1261 agagcttaca ccgagtaatg gtctgaggca aggcgagcat gagtgcctgg ctaagtgaaa
     1321 gtcatcgctg gcagagtgga tatgtacact taatttagtc tgtagtttta ttaaacttcc
     1381 ttattagagg aggccttatt ttcttattaa ccagaggtct ccatgctggc tgtttcatct
     1441 tgttgcctcc tctcttaccg tgtatcctct cagggaggtg aggataattg agaaagggct
     1501 aaaaacagct ctcccgagtt tgagaaggtt ttcagttgtc aatgcttaca taatttagag
     1561 aggactccaa agcattgtga aagaaagctc attatgcgtc tcagtctcct tccttgtctt
     1621 ttcttgtagt tgtctccata gtgggatgta catatcccta ggctagattt ggagttgggt
     1681 gagaagaaaa tattaaaaac ttcattttta tttcattctt ttttatttcc cattcttttt
     1741 catttctatt ttatatatgt tatgtaatat acacaaccaa tttttctaca gtaaagtatg
     1801 tatataattt ataagcaaat atacataaat ttggtggtat gcacaaaagt ttttaatttt
     1861 taagttttcg cagtgtgcag tttaaaaatt ttgtagaact tttgtcttat aggatttgtt
     1921 gtataggcat ggcagctttt gctaagggtg ggcgcgccga cccagctttc ttgtacaaag
     1981 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2041 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2101 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2161 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     2221 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2281 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2341 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2401 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2461 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2521 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2581 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2641 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2701 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2761 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2821 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2881 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2941 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     3001 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     3061 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     3121 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3301 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3541 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
