LOCUS       sequence-96529-e        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_20
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2445..2544)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2561..2580)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2562..2580)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2585..2601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2703..2722)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2714..3523
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2781..2800)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3670..4258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4159..4178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccca cgactagtgg ctcaaatctt tagaaatgag
      721 gggatgggtc tggtagagca ccccagattc aaggcgggtg gcttggggaa agcagggaga
      781 agggtctgga aactgctgct gagcaggagc ccccacaccc acagagcagg tgcagggagg
      841 cccatctgtc catccgtctg tctgtgggga ggcatggtga cgtttcaggc cggaggtaga
      901 agttttcacc caggaggtag agggtgaggc cggactgcac tccaagggga ggaggtggga
      961 gagcacagcc tggctgctgc caccaagctc cccagaagag gggacccctc cccccgaaca
     1021 ccagcacatg taggcagaaa gacttggcct gtggagccct cagcctccac gcccaccagc
     1081 agggactggg tggtgatgtc agcaatcccc acagaaagga gggctgcaca gccgtcccag
     1141 gagccagcac tgtccccgtg attctacgtg cagcctccac aagtgtgtcg catggaaaca
     1201 agcagtggag agccacatgt gtgtcacatc acgcctcgcc tcactcgtgc taaacctcgc
     1261 tgaccaggca gaaccaggca cgagctgcgt atcaaagttg ctggaaggag aaacatcaga
     1321 tccctaagct ggttacctgg caggcagggg ccctagaggg aaatggggca ggtgaaatgg
     1381 agatgtgtct ttgaaatgtc tttatttggc gggagaacgt cttcataggg tacttatacc
     1441 atgaaaaacc ctgtacacag actaattttg aagcaaacat gtgaggcgtt cacagaaagg
     1501 gttcaggaag acaagcaccc cagccgcaga gagcccgtgg gctgggggca cggggcatgg
     1561 cagccctctc ctggggatgg gcaggatggg gcacgtaggg cggaggaaga ggctcacatg
     1621 gtgtgcgtgt ttgggagcac cctgggcagc ctctcgagtg tccagagaga ggacaccgtc
     1681 ttgagccagc agggacagcc aagagcgccc ggggagaggc ctgggtggag acagaacaca
     1741 gcaggctgtg ggcgtggcca atgttgaggg acggaggtgg ccagaggcct ctcagtggtg
     1801 cccagggagc tggggacgag gggcctcatc cttcccctga gcccctccct tcaggcccag
     1861 ggagtggcct ggggcccagg agagcccgct ccgcgctcac agcctccgtt cccagacacg
     1921 cccgggcctg agcccccagg ctgcactgtg ccacagaaca gactccccgg cccctccctg
     1981 ctcgagaggt gaccaaggcc cagggtctcc cagcgagcaa gaggccattg aactgttgtt
     2041 ggctgtgcag tgccctgccc tcggggaggc ggcctctaag aagcacacac caccagcagg
     2101 aaccgggcag acagaggtgg gggatggaaa gcaggggaag tggccacagc caaaccagga
     2161 gggggaaggg agggggcctc accaaggaga ccctcacctg gggcctggag aatgagaagg
     2221 gtctggaggc taaggagagg ggtggatgct ccaggaggca gggccagccc atgtgagggt
     2281 cctgaggtag gaaccagagg aaaccggctc tggggcgaga agggcggcgc ccagggggcc
     2341 acaggggccg gcggcgaggg accctcagcc gaggctggta tctcgagaac agcagggatg
     2401 cctcaaaggt tctcgcccag caagtggaaa gggtgggcgc gccgacccag ctttcttgta
     2461 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2521 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2581 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2641 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2701 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2761 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2821 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2881 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2941 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3001 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3061 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3121 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3181 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3241 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3301 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3361 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3421 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3481 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3541 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3601 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3781 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     4081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
