LOCUS       sequence-96536-e        5065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_31
FEATURES             Location/Qualifiers
     source          1..5065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3190..3289)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3306..3325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3307..3325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3330..3346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3330..3346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3448..3467)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3459..4268
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3526..3545)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4415..5003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4904..4923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccgt gtttgagctc ccctgaaagt tggcttgtgc
      721 cacaaacacc ttgtaaagag caaataaagt catggcttca tcttagctgc tcttgaaatt
      781 catagatgag gcaggataca gggtattgtg gaagtgaaga gcatggactt gggcttccac
      841 aactagtaaa tggtaaagta ggattcagac tacctcagtt tgaatcctgc tttaccattt
      901 actagttgag tggccttgca caattcactt gacctctctg tgcctcagtt tcctcattaa
      961 aatatggtgg tactaatagt agagtttaga attgtaaaaa tgttggggag gagtgtgttt
     1021 gacagtggca caggttatac gactcaacaa atccacagtt atagttggag atttcaacac
     1081 ttctgtctta gtgattgatg ggacaagtag atagaaaaat cagtgtggat ataggagatc
     1141 tgagcaacct tatcaaccaa attgacttga ttcacattca tagaacacgc taccccacaa
     1201 cttagacaac acaattagac tgtccaccag atgattcagt gccaaagtta ctggaaggtt
     1261 attatttgtt tttggtctgt aattgctaag ttgatacaaa atgaaatttt ataacaattg
     1321 tagaaccaat aggacagtga ttcttgtttc actgtgtata tagcttatct tgagtttttt
     1381 tccttttcat agttctgtat ttacagcatt cagatacttt ctccctttgg gaagtgcaaa
     1441 tgaaggacat ctaccttgtt cgtccttctt gtactacttc cccaggaatg tgaggtgggc
     1501 aagggatgat gacagagatg ggaaagtgaa tttgaggatg ggcactgact ttcaaaaaat
     1561 attttcatat atgtttttat ttgatagcaa taattatgca ggggaagggg gtaggtcttt
     1621 ggccatgttg tcatggtatt acttgctcct catctgcttg tctttcactg aagtgctctg
     1681 ttctcagaaa ccatcacctg tttcttattc taggaaagtg gtggcaggtg atgtaatagt
     1741 taattgggta agacatccaa gattattttt cctacataca aaataggctt tttaggacct
     1801 attaagaata atagggcaaa caagatccta tacacaggaa aataaaaatt atataaaaag
     1861 aactattcat gagaagagtg taagataatg caagcacatg aagaaatcta cactggggtg
     1921 gatgaatcat taccctgact tcccctggga ttttgcttgc tgatgccctc ctgctgagat
     1981 agatgtgctt ttgcaaagca gaattggagg tgagtaaata agaagggggt tattcacttt
     2041 tatcatcaca gtaccccaca tgtcggttca aggaaattct aatgaaagtg ctgtgagttt
     2101 gtggaattag aacccaaatt actcttagaa ttaaaagaat gagagtaggg acagcaccag
     2161 ttgcttattt gatgaaacag aagaagcctg attgagatca tcttggtgca ttaggaaaac
     2221 agcctcaggg ctcccgctga caggacactt ttcccaagta ctattaatta taatgcgtta
     2281 ttagctaact gcaggcagtg gacatccttt gcacgttaac taaagtgcaa cagccgttta
     2341 gctagtgggg cctacttggt ctttaaaatt gtcttaatca ttagtgaatt cgttaaacta
     2401 taacgttaag agataaaaat aatcgtcgct gttgcttggg aacaagtcca aactgaaact
     2461 ggttttcaga ggttctgagt acaaatgttc ttgactgtgt agattgacgg gagctctaga
     2521 cccagccagc ctcaccccga catcttcagg gttacacaat atcctaagat cattgttatg
     2581 ggtttgattt ttactttgga gctgcagtag gatgtgaggc agggaagatg gtaccatggg
     2641 cttcagttct cagtcattca gaaatttaca gtgtagttgg caaatttttg aaactcatat
     2701 tcctttcagt gctttttttt ttgggccatt tcactgacta ttaatggctc cacctgggga
     2761 ggcctggaaa tgatgctgaa actcaatcaa gcctccttgg cagttctgga aagaggtttt
     2821 gagtgtcagt gcagaggcta tgaagagaag ctcaaattgt gctttaggat tatctctggg
     2881 ttttgatgat tggcatattc ttcttctgcc acaaattact gagacttaac agaacaaaca
     2941 agtttaattg gtttggtttt gattcatgtt ttgatgattg accacaagca gaaaacatgg
     3001 gaaaaaactt ctcgtcaagt gacctgagaa gagaagttca ttgcttttca ttttatccaa
     3061 atgttttaaa atgtttaaat ttaattaaaa aatttacata cattaaaatt tacacttttt
     3121 ggtatttcat tttatgagtt tggacaaata catggttcag caaaccacca ccaaagggtg
     3181 ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     3241 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     3301 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     3361 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     3421 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     3481 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     3541 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     3601 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     3661 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     3721 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     3781 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     3841 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     3901 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     3961 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     4021 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     4081 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     4141 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     4201 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     4261 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     4321 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4561 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4801 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5041 tttgctggcc ttttgctcac atgtt
//
