LOCUS       sequence-96539-e        3163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_34
FEATURES             Location/Qualifiers
     source          1..3163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1288..1387)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1404..1423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1405..1423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1428..1444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1428..1444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1546..1565)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1557..2366
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1624..1643)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2513..3101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3002..3021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcacccc acaaagaatg tgcaggaagg ggaggagctc
      721 aaaaatgagc aaggttgagg gtgggtatga gaagggggag aaggcgttct agagtggaga
      781 ggagggggtg aggtgtgact gcatggaaat aaaccagggc agggcccgct tgaagatctg
      841 aacttcagca tcgcaaagtg gtggcgtgag atgcagagag aggcaccaga tgctgatgtt
      901 tgttctctgt gtggcggctt agagaatgga gtggatggga gggaggccag gtgagatcct
      961 gcctgccagg caactgccta ttgtggttac catggaaaca ttagactgca gacccagcag
     1021 cctctagttc ggcccctctt attaaagatg acgagactga ggaccagaca ggggaagaga
     1081 tttgctcttt cctgctggtg gcctggaaac ctctgtccaa aaatactgca gtaaaacaca
     1141 cttaagataa aagtcactat ttactttgca accattttta tttattatta ttttagacag
     1201 aatccctctc tgtcacccaa gctagaatgc catcttggct cactgcaacc tctgccttct
     1261 gggctcaagc gaagggtggg cgcgccgacc cagctttctt gtacaaagtt ggcattataa
     1321 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1381 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1441 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1501 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     1561 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     1621 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     1681 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1741 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     1801 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     1861 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     1921 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     1981 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     2041 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     2101 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     2161 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     2221 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     2281 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     2341 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     2401 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     2461 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2641 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2881 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     2941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
