LOCUS       sequence-96543-e        4464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_39
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2589..2688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2705..2724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2706..2724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2729..2745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2729..2745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2847..2866)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2858..3667
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2925..2944)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3814..4402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4303..4322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccga tccaccagcc tcagtctccc aaagtgctgg
      721 gattacaggc atgagccact gtgcctggcc ctgaattatt tttattgtaa gtttcattct
      781 attcaaatat ttattttact gagtcaactc atttttgact caatgtattg ctatgtaaaa
      841 tgttcaacta gctaacaact taaaaaaatt agatcatgtg ctatctctat gcattgactt
      901 gggctgtctt ttcctctgcc tttgtgttta tttcattcct atatataact cttttggact
      961 tagagtcaca atggtttgaa gaaaatatgt ggaagaaaac tgacctgttg tttttatttt
     1021 taaatttgca gtatgcttct tcaatgtagc tcacctcagg gagcatttta gaagtctttt
     1081 cctggaatcc tatgaaccct atggcataga gttttataaa tgactgtttt ggtttgacta
     1141 gcatgggtcc acctgaacca gttctcactg taatgtccag gaagttgatt tgcttttctc
     1201 tgtctacaca gaagaaacac atgttgtgta gaaaagaaat tgccagtctc tgaacatttc
     1261 cctttgcagt gtctgatata gaaatctgta agcatttatg cagattctag aaatatttga
     1321 tctttcaata tttttgcaag tgtccaaacc taagtgatat gtttatatac attttctcat
     1381 ttaaaacaac taagtcacca gctaaggttg ccagcatttc gttgactatt tctagccgcc
     1441 aggctttgga aaggtcactc tacttcacat ggtagaggaa agtaaatagt tctgtgcagc
     1501 tgtcagcata gaggccttct cacttgatgt tattttcatc ctgtattgaa atgctctatt
     1561 tcctgtaaca ttaagttttc caaaatagag ggagagcaag gcatataata tttgtctgct
     1621 catgtcacct tacccaggct actgggcaca taagcatctc cagaggaaag cttcattagt
     1681 tgatagggaa acagtcacca atgttgtgtt tctctatctg tggatcattc actacaaagg
     1741 gaaaagctgc ttccctgtat ttgttactat ggagaatact gaagcaaaca aactagtaga
     1801 aattctcaat aagaaactca aatgcataat ggaaataact ttgttttttt catgaagaaa
     1861 aataattgtt accttgagta actatagtgc tttttgaatc tctcttttga atcgaatgcc
     1921 ccccatggag atgtagtaaa aatgaactac tgcttttgtg cctgagtggg ataagctgat
     1981 tttcgaagga gacatgcttg actttggtcc atgtcacact atttagaggg tgatggaaaa
     2041 gtagtacaat gcaacctctt tgaaccaatg aaatcactga ggactttatg ccctgggcat
     2101 gacacacttc tttcaaaaat gggatttgat aatgacattg acagtggtaa acaaaagtgt
     2161 ctcttcacta atttgaagac gagacaaacg atatttggaa caagaggtta gttccttatt
     2221 gcctaacaga attggggtgt atcaaatctt tactaactta gtcatctctg aatattttag
     2281 tctaggaaac caaaatatgt tcgtatcgac ctacgatatt tcttcagcta atgattactt
     2341 ctttcagata atataactgg ggatttattt gaaatttttc ctacttgcca gacataatgg
     2401 agattgaaaa aaataagtgt caatgaaacc ggaatgacaa aaaagtccta tgagaagtgt
     2461 cacgtaaagc agactgcttg tttcaaaata ttgaaagtgg aaaaaataca cgaaatggta
     2521 tgagcagtaa gatgtccaat tttgtgtaca aagggcatca tgtatgcaca caaagggtgg
     2581 gcgcgccgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2641 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2701 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2761 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     2821 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     2881 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2941 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     3001 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     3061 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     3121 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     3181 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     3241 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     3301 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3361 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     3421 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3481 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3541 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3601 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3661 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     3721 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3961 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4201 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4441 ttgctggcct tttgctcaca tgtt
//
