LOCUS       sequence-96551-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY-compatible lentiviral conditional RNAi delivery vector; 
            SFFV-driven expression of TetR and Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-X-PURO
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        335..736
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataat
      241 tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
      301 agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa
      361 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
      421 gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
      481 gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg
      541 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
      601 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
      661 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
      721 ccgacagact gagtcg
//
