LOCUS       sequence-96553-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY-compatible lentiviral conditional RNAi delivery vector; 
            SFFV-driven expression of TetR and Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-X-PURO
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11..631
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(84..103)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             632..652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             665..718
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      734..743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(746..765)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atccgccacc atggctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
       61 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
      121 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
      181 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
      241 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
      301 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
      361 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
      421 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
      481 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
      541 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
      601 agaaaaacaa cttaaatgtg aaagtgggtc gccaaaaaag aagagaaagg tcgacggcgg
      661 tggtgaaggc cgcggcagcc tgctgacctg cggcgatgtg gaagaaaacc cgggcccggg
      721 ggatccaccg gtcgccacca tggccatgac cgagtacaag cccacggtgc gcctcgccac
      781 ccgcgacgac gt
//
