LOCUS       sequence-96556-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven 
            Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-S-PURO
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(84..208)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        309..710
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
       61 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
      121 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      181 ctcgttcagc tttcttgtac aaagtggtga taattcctgc agccccgata aaataaaaga
      241 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
      301 tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga atagagaagt
      361 tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga tatctgcggt
      421 gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt tcggccccgg
      481 cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt ttcttaagac
      541 ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct tatttgaatt
      601 aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag ctctataaaa
      661 gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
//
