LOCUS       sequence-96561-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Splitposon in KanR ColE1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_1
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(385..404)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(469..488)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct gtggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata acgcgaagat
      241 ctgattgatt gaacgaaaaa cgcgaaagcg tttcacgata aatgcgaaaa cggatcctgt
      301 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
      361 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
      421 cttgttcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      481 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg gttgtatggg aagcccgatg
      541 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      601 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      661 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
      721 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgtt
//
