LOCUS       sequence-96566-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Triple split (at 67, 601-SZ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_14
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(121..126,127..142,143..149)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    155..174
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        190..239
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             263..274
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             486..497
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 cgcgccgctt cgtcaggcca catagctttc ttgttctgat cggaacgatc gttggctgtg
      121 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcgctcac aattctatgg
      181 actatgtttc tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc
      241 ctctacaaat aattttgttt aaaaagagga gaaatactag atgaacacga ttaacatcgc
      301 taagaacgac ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca
      361 ttacggtgag cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg
      421 tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa
      481 ctaataaaga ggagaaatac tagatgaata acgctgccgc caagcctctc atcactaccc
      541 tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg
      601 gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca
      661 tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg
      721 tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc
      781 ttgaagctaa gcacttcaag aaa
//
