LOCUS       sequence-96573-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PTac K1FR sigma fragment expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_25
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(120..125,126..141,142..148)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    154..173
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        189..238
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggc
       61 gcgccgcttc gtcaggccac atagctttct tgttctgatc ggaacgatcg ttggctgtgt
      121 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcgctcaca attctatgga
      181 ctatgtttct gtcaccggat gtgctttccg gtctgatgag tccgtgagga cgaaacagcc
      241 tctacaaata attttgttta atcacacagg aaagtactag atgagcatcg cggcgaccct
      301 ggagaacgat ctggcgcgtc tggaaaacga aaacgctcgt ctcgaaaaag acatcgcgaa
      361 cctggaacgt gacctggcga aactggagcg tgaagaagcg tacttcggag gttcaggtgg
      421 taagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
      481 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
      541 cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat
      601 tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa
      661 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
      721 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
      781 tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata
      841 caagaagcct attcagacgc gcttgaacct gaggttcctc ggttcgttca acctccagcc
      901 gaccgtcaac accaacaaag atagcgagat tgatgcacac aa
//
