LOCUS       sequence-96586-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable 
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes. This LBR contains N-terminal domain only..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-LBR-mRFP1-polyA
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
       61 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
      121 actccttgat gacgtcctcg gaggaggcca ttggatccgg gtccttctgc ctgcactgca
      181 gcagcagcaa gaggacgcag gcgggcaggc ccagcatgat gaggaccgca ccaggtactc
      241 ctccaaactc caagtccttc ctctgtggag tggtcacttg aaaggttccc aaaggtgcgg
      301 gtcctttggt gacaaggttt tgttcctcag actctatttc tttggctttg acctcttctc
      361 tcctgggacg aaggctgtac tgtgtaacta tatatctgtc ttctgttgac aaaatgattc
      421 tttcctgctt gtctttatac ggcgtagcgt tcttctccat gtgttcgggc tccccgttgt
      481 acacgctgac gctgtttccg aacggcttca acacaagcgg agtcagctta acttgcagaa
      541 tttccctcct catctccttc tccttcttct ccttcacgtc accctcatgg gaagcagaga
      601 cggatcttct ggagcctttc ggtgctctgc ccggggatcg ggaacgtgat cttgagcggc
      661 tgcggctgcg gctgccacgg cgtcgagagg gagagctgga aatcgagcca ctttttcttt
      721 gcttaaagga tt
//
