LOCUS       sequence-9660-ex        4106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-Myc-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      132..319
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(187..211)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        394..737
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             738..1436
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSNRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      1440..1637
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1741..1760)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1742..1760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1847..1864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2018..2606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2098..2117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2777..3568,3569..3637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3400..3419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3638..3742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3810..3828
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3866..3888)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3988..4007
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4088..4106
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4088..4105
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaac
       61 gggggaggcg ggggtggaga gcagaaactc atctcagaag aggatctgta gggcgcgcca
      121 cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
      181 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
      241 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
      301 ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag atctgtttag
      361 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
      421 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
      481 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
      541 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
      601 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
      661 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
      721 tccgaacata aacaaccatg gcagaaccag cccaaaaaaa gcaaaaacaa actgttcagg
      781 agcgcaaggc gtttatctcc cgtatcacta atgaaactaa aattcaaatc gctatttcgc
      841 tgaatggtgg ttatattcaa ataaaagatt cgattcttcc tgcaaagaag gatgacgatg
      901 tagcttccca agctactcag tcacaggtca tcgatattca cacaggtgtt ggctttttgg
      961 atcatatgat ccatgcgttg gcaaaacact ctggttggtc tcttattgtt gaatgtatcg
     1021 gtgacctgca cattgacgat caccatacta ccgaagattg cggtatcgca ttagggcaag
     1081 cgttcaaaga agcaatgggt gctgtccgtg gtgtaaaaag attcggtact gggttcgcac
     1141 cattggatga ggcgctatca cgtgccgtag tcgatttatc taatagacca tttgctgtaa
     1201 tcgaccttgg attgaagaga gagatgattg gtgatttatc cactgaaatg attccacact
     1261 ttttggaaag tttcgcggag gcggccagaa ttactttgca tgttgattgt ctgagaggtt
     1321 tcaacgatca ccacagaagt gagagtgcgt tcaaggcttt ggctgttgcc ataagagaag
     1381 ctatttctag caatggcacc aatgacgttc cctcaaccaa aggtgttttg atgtgaagta
     1441 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     1501 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     1561 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     1621 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct
     1681 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     1741 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     1801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     2221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3781 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3841 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3901 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3961 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4021 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     4081 acatacgatt taggtgacac tataga
//
