LOCUS       sequence-96603-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc Separase delta 55/Non-Cleavable in the
            genome and Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Holland AJ, Bottger F, Stemmann O, Taylor SS
  TITLE     Protein phosphatase 2A and separase form a complex regulated by 
            separase autocleavage.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29.
  PUBMED    17604273
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24623-32. 
            Epub 2007 Jun 29"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase  delta 55/Non-Cleav...
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     38..62
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             323..352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
       61 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      121 gcctccggac tctagcgttt aaacttaagc ttggtacccc ggggaattcg gcagatcccg
      181 aggtccgaca gcgcccggcc cagatcccca cgcctgccag gagcaagccg agagccagcc
      241 ggccggcgca ctccgactcc gagcagtctc tgtccttcga cccgagcccc gcgccctttc
      301 cgggacccct gccccgcgga tggagcagaa gctcatctca gaagaagacc tcgagaggag
      361 cttcaaaaga gtcaactttg ggactctgct aagcagccag aaggaggctg aagagttgct
      421 gcccgacttg aaggagttcc tgtccaaccc tccagctggt tttcccagca gccgatctga
      481 tgctgagagg agacaagctt gtgatgccat cctgagggct tgcaaccagc agctgactgc
      541 taagctagct tgccctaggc atctggggag cctgctggag ctggcagagc tggcctgtga
      601 tggctactta gtgtctaccc cacagcgtcc tcccctctac ctggaacgaa ttctctttgt
      661 cttactgcgg aatgctgctg cacaaggaag cccagaggcc acactccgcc ttgctcagcc
      721 cctccatgcc tgcttggtgc agtgctctcg cgaggctgct
//
