LOCUS       sequence-96611-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with N-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: C-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg gtgccaccat
       61 gggaaaaccg gaatttgccc caactacata tactgtcggc gtcccagggc tggttcgatt
      121 cctggaagcc caccacaggg atccagacgc tcaggcaatc gcagacgagc tgaccgacgg
      181 gaggttttat tatgctaagg tcgcctctgt gaccgatgca ggcgtacagc ctgtgtattc
      241 tcttagggtg gacacagccg accatgcttt tattaccaat ggcttcgtta gtcacaatac
      301 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
      361 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
      421 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
      481 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
      541 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
      601 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
      661 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
      721 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
      781 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
      841 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
      901 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
      961 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     1021 cgacaatctg accaaggccg agagaggcgg cctgagcgaa
//
