LOCUS       sequence-96626-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-emerin-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..130
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     370..391
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      433..442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
       61 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      121 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      181 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
      241 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      301 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      361 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      421 cgcccttgct caccatggtg gcgaccggtg gatccctggg atccatccag aaggggttgc
      481 cttcttcagc ttgtatggaa tagtaaacaa agagcagaaa ggcagcaaag accaggaaga
      541 gtaacagctg gccccagagt gggacctgtc gatcctggcc cagagcagca gcaggggcct
      601 gcttctctgg ccgaatggca cgtcgggtaa gccatgaaga gggagaagat gaagaggagg
      661 acacagaaga ggtggaggat gtaggataat atgataagcc cagagagctc ctggagacat
      721 tggaaa
//
