LOCUS       sequence-96627-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc hSeparase in the genome and 
            Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Holland AJ, Taylor SS
  TITLE     Cyclin-B1-mediated inhibition of excess separase is required for 
            timely chromosome disjunction.
  JOURNAL   J Cell Sci. 2006 Aug 15;119(Pt 16):3325-36. Epub 2006 Jul 25.
  PUBMED    16868023
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-08-15"; volume: "119(Pt 16)"; pages: "3325-36. 
            Epub 2006 Jul 25"
COMMENT     Sequence Label: pcDNA5 FRT TO myc hSeparase
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             327..356
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctcc ggactctagc gtttaaactt aagcttggta ccccggggaa ttcggcagat
      181 cccgaggtcc gacagcgccc ggcccagatc cccacgcctg ccaggagcaa gccgagagcc
      241 agccggccgg cgcactccga ctccgagcag tctctgtcct tcgacccgag ccccgcgccc
      301 tttccgggac ccctgccccg cggatggagc agaagctcat ctcagaagaa gacctcgaga
      361 ggagcttcaa aagagtcaac tttgggactc tgctaagcag ccagaaggag gctgaagagt
      421 tgctgcccga cttgaaggag ttcctgtcca accctccagc tggttttccc agcagccgat
      481 ctgatgctga gaggagacaa gcttgtgatg ccatcctgag ggcttgcaac cagcagctga
      541 ctgctaagct agcttgccct aggcatctgg ggagcctgct ggagctggca gagctggcct
      601 gtgatggcta cttagtgtct accccacagc gtcctcccct ctacctggaa cgaattctct
      661 ttgtcttact gcggaatgct gctgcacaag gaagcccaga ggccacactc cgccttgctc
      721 agcccctcca tgcctgcttg gtgcagtgct ctcgcgaggc tgctccccag gactatgagg
      781 ccgtggctc
//
