LOCUS       sequence-96630-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc Separase S1226A/ C2029A in the genome 
            and Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Holland AJ, Taylor SS
  TITLE     Cyclin-B1-mediated inhibition of excess separase is required for 
            timely chromosome disjunction.
  JOURNAL   J Cell Sci. 2006 Aug 15;119(Pt 16):3325-36. Epub 2006 Jul 25.
  PUBMED    16868023
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-08-15"; volume: "119(Pt 16)"; pages: "3325-36. 
            Epub 2006 Jul 25"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase S1226A/ C2029A
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             331..360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccccgg ggaattcggc
      181 agatcccgag gtccgacagc gcccggccca gatccccacg cctgccagga gcaagccgag
      241 agccagccgg ccggcgcact ccgactccga gcagtctctg tccttcgacc cgagccccgc
      301 gccctttccg ggacccctgc cccgcggatg gagcagaagc tcatctcaga agaagacctc
      361 gagaggagct tcaaaagagt caactttggg actctgctaa gcagccagaa ggaggctgaa
      421 gagttgctgc ccgacttgaa ggagttcctg tccaaccctc cagctggttt tcccagcagc
      481 cgatctgatg ctgagaggag acaagcttgt gatgccatcc tgagggcttg caaccagcag
      541 ctgactgcta agctagcttg ccctaggcat ctggggagcc tgctggagct ggcagagctg
      601 gcctgtgatg gctacttagt gtctacccca cagcgtcctc ccctctacct ggaacgaatt
      661 ctctttgtct tactgcggaa tgctgctgca caaggaagcc cagaggccac actccgcctt
      721 gctcagcccc tccatgcctg cttggtgcag tgctctcgcg aggctgct
//
