LOCUS       sequence-96635-e         383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High full length T7 RNAP* expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_41
FEATURES             Location/Qualifiers
     source          1..383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(5..10,11..28,29..35)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     48..70
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     67..83
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     67..83
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        101..119
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     101..118
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    126..182
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(184..203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(185..203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(210..227)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(210..226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(219..241)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tagctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
       61 ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa
      121 tactcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
      181 tctccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
      241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      301 cgtaatagcg aaaaggcccg caccgatcgc ccttcccaac agttgcgcat cctgaatggc
      361 gaatggacgc gccctgtagc ggc
//
