LOCUS       sequence-96639-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PTac alpha fragment expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_29
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(315..364)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     protein_bind    380..399
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(405..411,412..427,428..433))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(587..606)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agcctccggt cggaggcttt tgactttctg ctaatctaac taactaaccc ttagtgactc
       61 ctgcagcgtt agttatccgc aacctcacca gctttaagtt gacgctcaaa catcttgcgg
      121 aagcgtgctt cacccatctc gtaagactca tgctcaaggg ccaactgttc gcgagctaaa
      181 cgctcaccgt aatggtcagc cagagtgttg aacgggatag cagccagttc gatgtcagag
      241 aagtcgttct tagcgatgtt aatcgtgttc atctagtact ttcctgtgtg attaaacaaa
      301 attatttgta gaggctgttt cgtcctcacg gactcatcag accggaaagc acatccggtg
      361 acagaaacat agtccataga attgtgagcg ctcacaattc cacacattat acgagccgat
      421 gattaattgt caacacagcc aacgatcgtt ccgatcagaa caagaaagct atgtggcctg
      481 acgaagcggc gcgccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
      541 gaagagagtc aattcagggt ggtgaatatg aaaccagtaa cgttatacga tgtcgcagag
      601 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      661 gcgaaaacgc gggaaaa
//
