LOCUS       sequence-96643-e         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of OleD (DQ195536) under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-OleD
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..28
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(55..60,61..77,78..83)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(258..305)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     301..319
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(387..416)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattctc atgtttgaca
       61 gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca
      121 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
      181 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatccggat
      241 atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
      301 gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca
      361 gccggatctc agtcgacggc gctattcaga tcctcttctg agatgagttt ttgttcgggc
      421 ccaagcttct aattcccccc accgttggg
//
