LOCUS       sequence-96651-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PTac GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_34
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(474..505)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(592..612)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
       61 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcat
      361 ccatccagtc tattccatct ctagcagaaa tcatccttag cgaaagctaa ggattttttt
      421 tatctgatta ccgcctttga gtgagcccca atgataaccc caagggaagt tttagtcaaa
      481 agcctccggt cggaggcttt tgactttctg ctaatctaac taactaaccc ttagtgactc
      541 ctgcagcgtt atttgtagag ctcatccatg ccatgtgtaa tcccagcagc agttacaaac
      601 tcaagaagga ccatgtggtc acgcttttcg ttgggatctt tcgaaaggac agattgtgtc
      661 gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt attttgttga
      721 taatggtctg ctagttgaac ggaaccatct tcaacgttgt ggcgaatt
//
