LOCUS       sequence-96654-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of 89B1 (AT1G73880) under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  TITLE     Anderson Lab Glucosyltransferase plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-89B1
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gaaagtgaac gaggaaaaca acaagccgac aaagacccat
      181 gtcttaatct tcccatttcc ggcgcaaggt cacatgattc ccctcctcga cttcacccac
      241 cgccttgctc tccgcggcgg cgccgcctta aaaataaccg tcctagtcac tccaaaaaac
      301 cttccttttc tctctccgct tctctccgcc gtagttaaca tcgaaccact tatcctccct
      361 tttccctccc acccttcaat cccctccggc gtcgaaaacg tccaagactt acctccttca
      421 ggcttccctt taatgatcca cgcgcttggt aatctccacg cgccgcttat ctcttggatt
      481 acttctcacc cttctcctcc agtagccatc gtatctgatt tcttccttgg
//
