LOCUS       sequence-96659-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            chloramphenicol resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2SC
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        281..383
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(450..469)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggttctgtt aagtaactga acccaatgtc gttagtgacg cttacccgca aaaaaccccg
       61 cttcggcggg gttttttcgc tcttaagagg tcactgacct aacaaaaaaa aaaccccgcc
      121 cctgacaggg cggggttttt tttggtcttg agtggcagag tcagttatcg cgagcagtat
      181 gtaagtagat cctcagtgtc agctagggat aacagggtaa tcctggtgtc cctgttgata
      241 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      301 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      361 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      421 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      481 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      541 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct c
//
