LOCUS       sequence-96660-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            kanamycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2SK
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttacgccc cgccctgctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      181 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      241 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      301 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      361 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
      421 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      481 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      541 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
      601 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
      661 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
      721 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
      781 tttgccatgt ttcagaaaca actctggcgc atcgg
//
