LOCUS       sequence-96663-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            kanamycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2SK
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(360..379)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(444..463)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gcggttctgt taagtaactg aacccaatgt cgttagtgac gcttacccgc aaaaaacccc
       61 gcttcggcgg ggttttttcg ctcttaagag gtcactgacc taacaaaaaa aaaaccccgc
      121 ccctgacagg gcggggtttt ttttggtctt gagtggcaga gtcagttatc gcgagcagta
      181 tgtaagtaga tcctcagtgt cagctaggga taacagggta atctgatcct tcaactcagc
      241 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
      301 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      361 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
      421 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      481 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
      541 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
      601 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
      661 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
      721 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
      781 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
      841 taacggtttg gttgatgc
//
