LOCUS       sequence-96665-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid for amplification of a selection cassette that 
            includes two transcription terminator elements, an I-SceI site and a
            trimethoprim resistance gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pT2ST
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..36
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattaac ctataaaaat aggcgtatca cgaggccctt atctcaagag tggcagcggt
       61 tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cccgcaaaaa accccgcttc
      121 ggcggggttt tttcgctctt aagaggtcac tgacctaaca aaaaaaaaac cccgcccctg
      181 acagggcggg gttttttttg gtcttgagtg gcagagtcag ttatcgcgag cagtatgtaa
      241 gtagatcctc agtgtcagct agggataaca gggtaatcgg atagacggca tgcacgattt
      301 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
      361 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
      421 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
      481 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
      541 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
      601 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgagg acgtattgat
      661 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
      721 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
      781 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
      841 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
      901 gggttaagca gggcggggcg taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
//
