LOCUS       sequence-96668-e         254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the lambda Red recombinase under control of the 
            arabinose-inducible P(araB) promoter and I-SceI under control of the
            anhydrotetracycline-inducible P(tetA) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pSLTS
FEATURES             Location/Qualifiers
     source          1..254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             60..68
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggaa ttcgagctct
       61 aaggaggtta taaaaaatgg atattaatac tgaaactgag atcaagcaaa agcattcact
      121 aacccccttt cctgttttcc taatcagccc ggcatttcgc gggcgatatt ttcacagcta
      181 tttcaggagt tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg
      241 ggcgcgtcac tacc
//
