LOCUS       sequence-96670-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express ERK KTR mClover under CMV promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiCMV Puro DEST ERKKTRClover
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     278..299
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(602..626)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(710..734)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(731..782)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(760..780)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aacttcacct cggcgcgggt cttgtaggta ccgtcgtcct tgaaagagat ggtgcgctcc
       61 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      121 tagcggctga agcaggccac gccgtagccg aaggtggtca cgagggtggg ccagggcacg
      181 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgttggt ggcatcgccc
      241 tcgccctcgc cgcggacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      301 atgggcacaa ccccggtgaa cagctcctcg cccttgctca ccatactagt ggatgggaat
      361 tgaaagctgg actggagggt caggcgctcc agccttgacg acaccgatgg ggtacgtggc
      421 ttcttcgatg gcgtcgctgg gtcctcgaga ccgctacgct tgctgggcga aagcgctggc
      481 cccggtgcct gaagaccaga gcttgttcct gatcctggag tccgttcagg tccctggcca
      541 cccagcaggc ttgggctaag tggaagttcc aggtcccgag gctttcggcc cttcatggtg
      601 gagcctgctt ttttgtacaa acttgttgat atctgcagaa ttccaccaca ctggactaga
      661 gtcggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
      721 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      781 tca
//
