LOCUS       sequence-96675-e         484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the lambda Red recombinase under control of the 
            arabinose-inducible P(araB) promoter and I-SceI under control of the
            anhydrotetracycline-inducible P(tetA) promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Kim J, Webb AM, Kershner JP, Blaskowski S, Copley SD
  TITLE     A versatile and highly efficient method for scarless genome editing 
            in Escherichia coli and Salmonella enterica.
  JOURNAL   BMC Biotechnol. 2014 Sep 25;14(1):84.
  PUBMED    25255806
REFERENCE   2  (bases 1 to 484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-09-25"; volume: "14(1)"; pages: "84"
COMMENT     Sequence Label: pSLTS
FEATURES             Location/Qualifiers
     source          1..484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..109
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    60..78
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    90..108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt tttgttgaca
       61 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatgc
      121 atatgaaaaa catcaaaaaa aaccaggtaa tgaacctggg tccgaactct aaactgctga
      181 aagaatacaa atcccagctg atcgaactga acatcgaaca gttcgaagca ggtatcggtc
      241 tgatcctggg tgatgcttac atccgttctc gtgatgaagg taaaacctac tgtatgcagt
      301 tcgagtggaa aaacaaagca tacatggacc acgtatgtct gctgtacgat cagtgggtac
      361 tgtccccgcc gcacaaaaaa gaacgtgtta accacctggg taacctggta atcacctggg
      421 gcgcccagac tttcaaacac caagctttca acaaactggc taacctgttc atcgttaaca
      481 acaa
//
