LOCUS       sequence-96678-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiCMV Puro DEST p38KTRmCerulean3
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     384..405
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(645..669)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(753..777)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gttgccgtgg atggcgttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
       61 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      121 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      181 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      241 ctgcacgccc cagctcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      301 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      361 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      421 ggtgaacagc tcctcgccct tgctcaccat actagtgctg gactggaggg tcaggcgctc
      481 cagccttgac gacacctccg gcgacgccag cttcttagat ggcgactcgt cttcgttgag
      541 accggccgag cgcttggagt tatttatcct ttgattcact gatggcatcg tgttcttgct
      601 gccaggtggg ataagaacgc ggagatctgg cttacgcatg gtggagcctg cttttttgta
      661 caaacttgtt gatatctgca gaattccacc acactggact agagtcggtg tcttctatgg
      721 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg
      781 cttatataga cctcccaccg taca
//
