LOCUS       sequence-96679-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of Myc ArC K72R in the genome and 
            Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Girdler F, Gascoigne KE, Eyers PA, Hartmuth S, Crafter C, Foote KM, 
            Keen NJ, Taylor SS
  TITLE     Validating Aurora B as an anti-cancer drug target.
  JOURNAL   J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.
  PUBMED    16912073
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-09-1"; volume: "119(Pt 17)"; pages: "3664-75. 
            Epub 2006 Aug 15"
COMMENT     Sequence Label: pcDNA5 FRT TO Myc  ArC K72R
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             331..360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccccgg ggaattcggc
      181 agatcccgag gtccgacagc gcccggccca gatccccacg cctgccagga gcaagccgag
      241 agccagccgg ccggcgcact ccgactccga gcagtctctg tccttcgacc cgagccccgc
      301 gccctttccg ggacccctgc cccgcggatg gagcagaagc tcatctcaga agaagacctc
      361 gagggatcca gctcccccag agctgtggtg cagctgggca aagctcaacc tgcaggcgaa
      421 gagttggcta cagcaaacca aacagcccag cagcccagca gcccagccat gcggcgcctc
      481 acagtcgatg actttgaaat cgggcgtccc ctgggcaagg ggaaatttgg gaatgtgtac
      541 ctggctcggc tcaaggaaag ccatttcatt gtggccctgc gggttctctt caagtcgcag
      601 atagagaagg aaggactgga gcaccagctg cgccgggaaa ttgagatcca ggctcatcta
      661 caacacccca atatcctgcg cctgtataac tatttccatg atgcacgccg ggtgtacctg
      721 attctggaat atgctccaag gggtgagctc tacaaggagc tgcag
//
