LOCUS       sequence-96682-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc Separase Non-Cleavable in the genome 
            and Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Holland AJ, Bottger F, Stemmann O, Taylor SS
  TITLE     Protein phosphatase 2A and separase form a complex regulated by 
            separase autocleavage.
  JOURNAL   J Biol Chem. 2007 Aug 24;282(34):24623-32. Epub 2007 Jun 29.
  PUBMED    17604273
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-08-24"; volume: "282(34)"; pages: "24623-32. 
            Epub 2007 Jun 29"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase Non-Cleavable
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             331..360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccccgg ggaattcggc
      181 agatcccgag gtccgacagc gcccggccca gatccccacg cctgccagga gcaagccgag
      241 agccagccgg ccggcgcact ccgactccga gcagtctctg tccttcgacc cgagccccgc
      301 gccctttccg ggacccctgc cccgcggatg gagcagaagc tcatctcaga agaagacctc
      361 gagaggagct tcaaaagagt caactttggg actctgctaa gcagccagaa ggaggctgaa
      421 gagttgctgc ccgacttgaa ggagttcctg tccaaccctc cagctggttt tcccagcagc
      481 cgatctgatg ctgagaggag acaagcttgt gatgccatcc tgagggcttg caaccagcag
      541 ctgactgcta agctagcttg ccctaggcat ctggggagcc tgctggagct ggcagagctg
      601 gcctgtgatg gctacttagt gtctacccca cagcgtcctc ccctctacct ggaacgaatt
      661 ctctttgtct tactgcggaa tgctgctgca caaggaagcc cagaggccac actccgcctt
      721 gctcagcccc tccatgcctg cttggtgcag tgctctcgcg aggctgctcc ccaggactat
      781 gaggccgtgg ctc
//
