LOCUS       sequence-96690-e         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express PKA KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..503)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(52..71)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     445..468
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
       61 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
      121 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
      181 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
      241 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
      301 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
      361 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
      421 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
      481 tgggaaaagc gcctccccta cccggtagaa ttccaggcgg ggaggcggcc caaagggaga
      541 tccgactcgt ctgagggcga aggcgaa
//
