LOCUS       sequence-96745-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for sgRNA cloning (F+E modified backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>sgRNA(F+E)
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 taggcgtatc acgaggccct tacgtattaa ttaaggcgcg cctggcgggt gtattaaacc
       61 actaaacaaa caattgcccc aagctctctt cacaattata aacactacaa atgtttggac
      121 aagagattag cgtggctgtg acgagtaatc tcaaaggctt ggtgtaattg atattttata
      181 agaagcagat taaacttcaa tacagttaac acctcattta caaaaaattg gctgccaaaa
      241 tcgctaatta acacatattt aaaacaattt ctacagatat acacagtata gtatgattac
      301 taactgcata ataaacaaac atatccaaca gacactcact aatctgccat aacaagcttc
      361 aaaaacttaa actcgaaatt ttagtgaatc ttttttttta atgaagattt tatttaaaaa
      421 gttaaaaata ttacagttca ggtataggtt tacacctaat ctttaataat ccgaactaaa
      481 ttttaactat ttagaaactt tttcaaccaa agtttaaaaa aatagattct tcgcacgcta
      541 aaactatcat ttacacaaaa aaatgcaaca aaatgcagaa aaaaattaca ttagagttta
      601 ggttagttac ctgctaatca ttataaacta acttccggca taatattcat ctaaaattag
      661 caataatcac gttttacgct aaaatttgtg taaaactaaa cttcgtcctt tgtcaaggag
      721 aaaatttgac tcaaaagctg cgcgcgca
//
