LOCUS       sequence-96750-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian 
            M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF
  TITLE     High frequency targeted mutagenesis in Arabidopsis thaliana using 
            zinc finger nucleases.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 29. 107(26):12028-33.
  PUBMED    20508152
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 29. 107(26):12028-33."
COMMENT     Sequence Label: pFZ19
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             380..685
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     564..583
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(726..850)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc ggcttactaa
       61 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
      121 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
      181 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
      241 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
      301 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
      361 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
      421 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
      481 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
      541 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
      601 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
      661 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      721 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      781 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      841 acaaagtggt tcgataattc ttaattaact agtcgatcca ggcctcccag ctttcgtccg
      901 tatcatcggt ttcga
//
