LOCUS       sequence-96754-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian 
            M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF
  TITLE     High frequency targeted mutagenesis in Arabidopsis thaliana using 
            zinc finger nucleases.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 29. 107(26):12028-33.
  PUBMED    20508152
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 29. 107(26):12028-33."
COMMENT     Sequence Label: pFZ19
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(333..379)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtttgata gcttggcgcg
      301 cctcgaggct agagtcgact agcttcagcg tgtcctctcc aaatgaaatg aacttcctta
      361 tatagaggaa gggtcttgcg aagatcctag agtcgagcat attacatggc attaccacca
      421 tatacatatc catatacata tccatatcta atcttacctc gactgctgta tataaaacca
      481 gtggttatat gtacagtact gctgtatata aaaccagtgg ttatatgtac agtacgtcga
      541 ctgctgtata taaaaccagt ggttatatgt acagtactgc tgtatataaa accagtggtt
      601 atatgtacag tacgtcgagg ggatgataat gcgattagtt ttttagcctc gacctgcagc
      661 ccaagcggca tgcaagctgt cgagggggga tcaattcccg atctagtaac atagatgaca
      721 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
      781 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
      841 tgttaattat tacatgctta acgtaattca
//
