LOCUS       sequence-96758-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for sgRNA cloning (F+E modified backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>sgRNA(F+E)
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttccgagtt tgtcagaaag cagaccaaac agcggttgga ataatagcga gaacagagaa
       61 atagcggcaa aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa
      121 atcgggaaaa acgggaagta ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg
      181 aacatccaaa agtttgtgtt ttttaaatag tacataatgg atttccttac gcgaaatacg
      241 ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag
      301 cgaccggcgc tcagctggaa ttcaaaaaaa gcaccgactc ggtgccactt tttcaagttg
      361 ataacggact agccttattt aaacttgcta tgctgtttcc agcatagctc ttaaactgag
      421 accctctctc ggtctctatc tataccatcg gatgccttcc agacttataa gcgtgcttaa
      481 gtctagttct caatttaaag gggaaatacc gtctgtgtac gcagagacac tgtctgtacg
      541 ttatttgtgc gtttttgggc tcgcgcgatc catttttggc gtttttagac ataaaaagcg
      601 gtttatttgc ccccaaaatt aaaaaatatg tatttttact tattagggga tttttccaca
      661 tgattatgat gtaacaaaca ctcgcttggg gatctcccct gcgcgcgcag cttttgagtc
      721 aaattttctc cttgac
//
