LOCUS       sequence-96759-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eef1a (EF1alpha) promoter driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Eef1a-1955/-1>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(333..353)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttccgagtt tgtcagaaag cagaccaaac agcggttgga ataatagcga gaacagagaa
       61 atagcggcaa aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa
      121 atcgggaaaa acgggaagta ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg
      181 aacatccaaa agtttgtgtt ttttaaatag tacataatgg atttccttac gcgaaatacg
      241 ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag
      301 cgaccggcgc tcagctggaa ttctcattaa cctaccttgc gctttttctt gggagctccc
      361 tcatcccctc cgagctgtga gaggtcaatc cgagtttcgt aaagtccggt gatgctctgg
      421 tggatgaggg tggcgtccag cacttctttg gtggaggtat agcgtttccg gtcgatggtg
      481 gtgtcgaagt acttgaatgc ggcaggggca ccaagattgg tgagggtgaa caggtggatg
      541 atattctcag cctgctcccg aattggtttg tctctgtgct tattgtatgc gctaaggact
      601 ttatcgaggt tggcatcagc gagaatgacg cgtttagaaa actcggaaat ctgctcaatg
      661 atctcgtcca gataatgctt atgctgctcc acaaacagtt gcttttgctc gttgtcctcg
      721 gggctcccct tcagcttctc gtaatggctt gcaaggtaga ggaaattcac gtacttggag
      781 gggagagcca gctcattacc cttctgaagt tcgccagcgg aggcgagcat ccgcttccg
//
