LOCUS       sequence-96764-e        4029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a TALEN protein specific for mouse ROSA26 locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Kasparek P, Krausova M, Haneckova R, Kriz V, Zbodakova O, Korinek V,
            Sedlacek R
  TITLE     Efficient gene targeting of the Rosa26 locus in mouse zygotes using 
            TALE nucleases.
  JOURNAL   FEBS Lett. 2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014.
  PUBMED    25241166
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2014 Sep 18. pii: S0014-5793(14)00689-9. doi: 
            10.1016/j.febslet.2014.09.014."
COMMENT     Sequence Label: TALEN-mROSA26 KKR
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    771..892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(782..801)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     836..855
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1025..1129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1130..1198,1199..1990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1348..1367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2161..2749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2650..2669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2878..3235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2878..3069
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2901..2921)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3086..3221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3305..3437
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3420..3439
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3482..3500
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3504..3513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3513..3578
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3585..3605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacgaccacc tcgtagcgct ggcctgcctc ggcggacgac ccgccttgga tgcggtgaag
       61 aaggggctcc cgcacgcgcc tgcattgatt aagcggacca acagaaggat tcccgagagg
      121 acatcacatc gagtggcagg ttcccaactc gtgaagagtg aacttgagga gaaaaagtcg
      181 gagctgcggc acaaattgaa atacgtaccg catgaataca tcgaacttat cgaaattgct
      241 aggaactcga ctcaagacag aatccttgag atgaaggtaa tggagttctt tatgaaggtt
      301 tatggatacc gagggaagca tctcggtgga tcacgaaaac ccgacggagc aatctatacg
      361 gtggggagcc cgattgatta cggagtgatc gtcgacacga aagcctacag cggtgggtac
      421 aatcttccca tcgggcaggc agatgagatg caacgttatg tcaaagaaaa tcagaccagg
      481 aacaaacaca tcaatccaaa tgagtggtgg aaagtgtatc cttcatcagt gaccgagttt
      541 aagtttttgt ttgtctctgg gcatttcaaa ggcaactata aggcccagct cacacggttg
      601 aatcgcaaaa cgaactgcaa tggtgcggtt ttgtccgtag aggaactgct cattggtgga
      661 gaaatgatca aagcgggaac tctgacactg gaagaagtca gacgcaagtt taacaatggc
      721 gagatcaatt tccgctcata atctagagcg gccgcttcga gcagacatga taagatacat
      781 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      841 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      901 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      961 gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt ttcggggaaa
     1021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
     2821 ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     2881 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2941 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3241 tgattcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg
     3301 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
     3361 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     3421 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
     3481 ttaatacgac tcactatagg ctagccacca tggactataa ggaccacgac ggagactaca
     3541 aggatcatga tattgattac aaagacgatg acgataagat ggccccaaag aagaagcgga
     3601 aggtcggtat ccacggagtc ccagcagccg tagatttgag aactttggga tattcacagc
     3661 agcagcagga aaagatcaag cccaaagtga ggtcgacagt cgcgcagcat cacgaagcgc
     3721 tggtgggtca tgggtttaca catgcccaca tcgtagcctt gtcgcagcac cctgcagccc
     3781 ttggcacggt cgccgtcaag taccaggaca tgattgcggc gttgccggaa gccacacatg
     3841 aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg agcgcttgag gccctgttga
     3901 cggtcgcggg agagctgaga gggcctcccc ttcagctgga cacgggccag ttgctgaaga
     3961 tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca cgcgtggcgc aatgcactga
     4021 cgggtgccc
//
