LOCUS       sequence-96767-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driving Ebf.774 (F+E) sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>Ebf.774(F+E)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttccgagtt tgtcagaaag cagaccaaac agcggttgga ataatagcga gaacagagaa
       61 atagcggcaa aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa
      121 atcgggaaaa acgggaagta ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg
      181 aacatccaaa agtttgtgtt ttttaaatag tacataatgg atttccttac gcgaaatacg
      241 ggcagacatg gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag
      301 cgaccggcgc tcagctggaa ttcaaaaaaa gcaccgactc ggtgccactt tttcaagttg
      361 ataacggact agccttattt aaacttgcta tgctgtttcc agcatagctc ttaaaccctg
      421 ttgtccaacc ctcagcatct ataccatcgg atgccttcca gacttataag cgtgcttaag
      481 tctagttctc aatttaaagg ggaaataccg tctgtgtacg cagagacact gtctgtacgt
      541 tatttgtgcg tttttgggct cgcgcgatcc atttttggcg tttttagaca taaaaagcgg
      601 tttatttgcc cccaaaatta aaaaatatgt atttttactt attaggggat ttttccacat
      661 gattatgatg taacaaacac tcgcttgggg atctcccctg cgcgcgcagc ttttgagtca
      721 aattttctcc ttgacaaagg acgaag
//
