LOCUS       sequence-96775-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: Empty sgRNA target (CspCI placeholder) (see 
            comments)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ374
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..400
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     misc_RNA        519..570
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(620..637)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(620..636)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..651)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctatgacatg attacgaatt cgagctcggt accccgggta ccttttgctt atgttggtgg
       61 tagttggcat gcgtagactg atgactagtc agcaaggagc gtagaacagt cacactcgtt
      121 atatatgtgc ttccaagaaa actcaagaat ttaccattag caaacacttt tttgaaatgt
      181 tagacattta aatgacgaag gcatatagaa gctttgaata ggtgttgtaa agtgttgatt
      241 tatgtgacgc tgagggtgcg catgaaagga atgttgggtc acgattatta aacagtttgc
      301 tagcttggac acttgagtat tggaagttgt tgaattctaa aaaactttca gttgatttga
      361 atagttgctg ttgccaaaaa acataacctg taccgaagaa tgggcttaac tcaattcttg
      421 tgggttatct ctctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      481 caacttgaaa aaagtgagtg gcaccgagtc ggtggtgccc tgtcaccgga tgtgctttcc
      541 ggtctgatga gtccgtgagg acgaaacagg ggtaccgtgg ggatcctcta gagtcgacct
      601 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      661 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      721 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      781 tgatgcggta
//
