LOCUS       sequence-96780-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian 
            M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF
  TITLE     High frequency targeted mutagenesis in Arabidopsis thaliana using 
            zinc finger nucleases.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 29. 107(26):12028-33.
  PUBMED    20508152
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 29. 107(26):12028-33."
COMMENT     Sequence Label: pFZ19
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..52
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    101..225
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(250..255,256..273,274..280)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(400..419)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacacgctga
       61 agctagtcga ctctagcctc gaggcgcgcc aagctatcaa acaagtttgt acaaaaaagc
      121 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      181 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
      241 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
      301 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      361 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      421 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      481 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      541 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      601 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      661 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      721 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      781 tgggtgagtt tcaccagttt tgatt
//
