LOCUS       sequence-96781-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian 
            M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF
  TITLE     High frequency targeted mutagenesis in Arabidopsis thaliana using 
            zinc finger nucleases.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 29. 107(26):12028-33.
  PUBMED    20508152
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 29. 107(26):12028-33."
COMMENT     Sequence Label: pFZ19
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      186..438
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        759..805
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    854..878
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
       61 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
      121 cagcactcgt ccgggatctt ggaggtgatg taacatgatc acaagctgat cccccgaatt
      181 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
      241 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
      301 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
      361 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
      421 catctatgtt actagatcgg gaattgatcc cccctcgaca gcttgcatgc cgcttgggct
      481 gcaggtcgag gctaaaaaac taatcgcatt atcatcccct cgacgtactg tacatataac
      541 cactggtttt atatacagca gtactgtaca tataaccact ggttttatat acagcagtcg
      601 acgtactgta catataacca ctggttttat atacagcagt actgtacata taaccactgg
      661 ttttatatac agcagtcgag gtaagattag atatggatat gtatatggat atgtatatgg
      721 tggtaatgcc atgtaatatg ctcgactcta ggatcttcgc aagacccttc ctctatataa
      781 ggaagttcat ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgaggcgc
      841 gccaagctat caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
      901 tatcaatata ttaaattaga ttttgc
//
