LOCUS       sequence-96783-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses PDZ2 domain of human ZO-1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Fanning AS, Lye MF, Anderson JM, Lavie A
  TITLE     Domain swapping within PDZ2 is responsible for dimerization of ZO 
            proteins.
  JOURNAL   J Biol Chem. 2007 Dec 28;282(52):37710-6. Epub 2007 Oct 9.
  PUBMED    17928286
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2007-12-28"; volume: "282(52)"; pages: "37710-6. Epub
            2007 Oct 9"
COMMENT     Sequence Label: pGEX-2T_PDZ2
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(313..335)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(480..498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        566..670
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctggttccgc gtggatccaa agtcacactg gtgaaatccc ggaaaaatga agaatatggt
       61 cttcgattgg caagccatat atttgttaag gaaatttcac aagatagttt ggcagcaaga
      121 gatggcaata ttcaagaagg tgatgttgta ttgaagataa atggtactgt gacagaaaat
      181 atgtcattga cagatgcaaa gacattgata gaaaggtcta aaggcaaatt aaaaatggta
      241 gttcaaagag attggaattc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga
      301 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      361 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      421 cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg
      481 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      721 ttgccttcct g
//
