LOCUS       sequence-96791-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APPL1 Endosomal Localization Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Broussard JA, Lin WH, Majumdar D, Anderson B, Eason B, Brown CM, 
            Webb DJ
  TITLE     The endosomal adaptor protein APPL1 impairs the turnover of leading 
            edge adhesions to regulate cell migration.
  JOURNAL   Mol Biol Cell. 2012 Apr;23(8):1486-99. doi: 10.1091/mbc.E11-02-0124.
            Epub 2012 Feb 29.
  PUBMED    22379109
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-02-0124"; journalName: "Mol Biol Cell"; date: 
            "2012-04"; volume: "23"; issue: "8"; pages: "1486-99"
COMMENT     Sequence Label: GFP-APPL1-R146A/K152A/R154A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(93..114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(354..373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     701..722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagctg tttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      241 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      301 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      481 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      541 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      601 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      661 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      721 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtactca gatctcgagc
      781 tcaagcttcg aattcggctt atgccgggga tcgacaagct gcccatcgag gagacgctgg
      841 aggacagccc gcagacaagg tctttactag gtgtatttga agaagatgcc acagctattt
      901 ccaactatat gaaccagttg tatcaagcta tgcatcggat ttatgatgca cagaatgaat
      961 taagtgca
//
