LOCUS       sequence-96792-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APPL1 Endosomal Localization Mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Broussard JA, Lin WH, Majumdar D, Anderson B, Eason B, Brown CM, 
            Webb DJ
  TITLE     The endosomal adaptor protein APPL1 impairs the turnover of leading 
            edge adhesions to regulate cell migration.
  JOURNAL   Mol Biol Cell. 2012 Apr;23(8):1486-99. doi: 10.1091/mbc.E11-02-0124.
            Epub 2012 Feb 29.
  PUBMED    22379109
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-02-0124"; journalName: "Mol Biol Cell"; date: 
            "2012-04"; volume: "23"; issue: "8"; pages: "1486-99"
COMMENT     Sequence Label: GFP-APPL1-R146A/K152A/R154A
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(63..266)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(92..112)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        267..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(825..844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tagcgctagc ggatctgacg gttcactaaa
       61 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      121 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      181 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      241 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
      301 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
      361 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
      421 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
      481 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
      541 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
      601 gaccccgtaa ttgattacta ttaataacta atgcatggcg gtaatacggt tatccacaga
      661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      961 cagttcggtg taggtcgttc gctcca
//
