LOCUS       sequence-96793-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APPL1 with PTB domain deleted .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Broussard JA, Lin WH, Majumdar D, Anderson B, Eason B, Brown CM, 
            Webb DJ
  TITLE     The endosomal adaptor protein APPL1 impairs the turnover of leading 
            edge adhesions to regulate cell migration.
  JOURNAL   Mol Biol Cell. 2012 Apr;23(8):1486-99. doi: 10.1091/mbc.E11-02-0124.
            Epub 2012 Feb 29.
  PUBMED    22379109
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-02-0124"; journalName: "Mol Biol Cell"; date: 
            "2012-04"; volume: "23"; issue: "8"; pages: "1486-99"
COMMENT     Sequence Label: GFP-APPL1-Delta-PTB
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(94..115)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(355..374)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     702..723
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggtagct gttcaccggg gctggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      541 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgag
      781 ctcaagcttc gaattcggct tatgccgggg atcgacaagc tgcccatcga ggagacgctg
      841 gaggacagcc cgcagacaag gtctttacta ggtgtatttg aagaagatgc cacagctatt
      901 tccaactata tgaaccagtt gtatcaagct atgcatcgga tttatgatgc acagaatgaa
      961 ttaagtgcag caacacacct gacctcaaaa cttttaaaag aatatgaaaa acagcgtttt
     1021 ccattgggag gtgatgatga agttatgagc tctacattg
//
