LOCUS       sequence-96799-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Folding reporter with ampicillin resistance as a readout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-EcoBam
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..90
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(105..110,111..128,129..135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             440..818
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(474..493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
       61 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      121 ccggctcgta tgttgtgtgg aattgtgagc gggataacaa tttcacacag gatctcgaca
      181 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
      241 taaccgtcgc cgggatgctg gggccgtcat tgctaacgcc gcgacgtgcg actgcgcata
      301 tggaattctc cagacgacgg atcccaccca gaaacgctgg tgaaagtaaa agatgctgaa
      361 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      421 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      481 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
      541 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      601 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      661 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      721 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      781 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
      841 ctacttactc tagc
//
