LOCUS       sequence-96801-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT with Ham1 insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-Ham1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..90
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(105..110,111..128,129..135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             813..824
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
       61 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      121 ccggctcgta tgttgtgtgg aattgtgagc gggataacaa tttcacacag gatctcgaca
      181 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
      241 taaccgtcgc cgggatgctg gggccgtcat tgctaacgcc gcgacgtgcg actgcgcata
      301 tggaattcat gaaattgtta gtcgcatcca ataacgccaa gaaactggga gaattgcaac
      361 gtatcttaga ccaggccgga attgaaaacg tggaactgct agcactagcc gacgtcccga
      421 gctatcctga gccagtggag gacggacgta cctttaccga gaacgccttg atcaaagcgc
      481 gtgcaggcgc aagcaataca gggttgatca cactggccga tgactcgggt ctagaggtcg
      541 atgctctaaa tggcatgcca ggcgtgcttt ctgcccgttg ggcaggcaaa catggaaacg
      601 accaagcaaa taatgaccta ttattggctc agatagctga tatccccgag gagcacagag
      661 gggcagcctt cgtcagtgtt tgtgctattg tcacacccga tggcagggaa tttgttgagg
      721 aaggcagatg gcatggtact ctactgagag aaccggtagg taccaatggg tttggatatg
      781 atccactttt cgtccccatg gaagaatcat tgatcgaggg acgtgacagg tcaagtgcgc
      841 aattgactgc acaag
//
